Article
Point mutations in awdKpn which revert the prune/Killer of prune lethal interaction affect conserved residues that are involved in nucleoside diphosphate kinase substrate binding and catalysis.
The Journal of biological chemistry - 29 Sept 1995
Timmons L, Xu J, Hersperger G, Deng X F, Shearn A
Abstract excerpt
The awd gene of Drosophila melanogaster encodes a nucleoside diphosphate kinase. Killer of prune (Kpn) is a mutation in the awd gene which substitutes Ser for Pro at position 97 and causes dominant lethality in individuals that do not have a functional prune gene. This lethality is not due to an...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Binding Sites
- Catalysis
- Chromatography, Affinity
- Codon
- Conserved Sequence
- DNA Primers
- Drosophila
- Drosophila Proteins
- Electrophoresis, Polyacrylamide Gel