Article
A Single Deoxynucleoside Kinase Variant from Drosophila melanogaster Synthesizes Monophosphates of Nucleosides That Are Components of an Expanded Genetic System.
ACS synthetic biology - 17 Mar 2017
Matsuura Mariko F, Winiger Christian B, Shaw Ryan W, Kim Myong-Jung, Kim Myong-Sang, Daugherty Ashley B, Chen Fei, Moussatche Patricia, Moses Jennifer D, Lutz Stefan, Benner Steven A
Abstract excerpt
Deoxynucleoside kinase from D. melanogaster (DmdNK) has broad specificity; although it catalyzes the phosphorylation of natural pyrimidine more efficiently than natural purine nucleosides, it accepts all four 2'-deoxynucleosides and many analogues, using ATP as a phosphate donor to give the corresponding deoxynucleoside monophosphates. Here, we show that replacing a single amino acid (glutamine 81 by glutamate)...
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