A Pro/Ser substitution in nucleoside diphosphate kinase of Drosophila melanogaster (mutation killer of prune) affects stability but not catalytic efficiency of the enzyme.

Abstract excerpt

Nucleoside diphosphate kinase of Drosophila, recently identified as the product of the awd gene, is essential for larval development. The conditional lethal mutation Killer of prune maps to the same gene. We purified the nucleoside diphosphate kinases from wild-type and mutant larvae by a simple procedure involving affinity chromatography on blue Sepharose. Both proteins are purified as hexamers in their native...

Topics

• Amino Acids
• Animals
• Catalysis
• Chromatography, Gel
• Drosophila Proteins
• Drosophila melanogaster
• Enzyme Activation
• Enzyme Stability
• Insect Hormones
• Isoelectric Focusing