Article
Cloning and characterization of the Saccharomyces cerevisiae LYS7 gene: evidence for function outside of lysine biosynthesis.
Gene - 30 Aug 1995
Horecka J, Kinsey P T, Sprague G F
Abstract excerpt
The Saccharomyces cerevisiae LYS7 gene has been cloned based on its genetic map position and complementation of a lys7 mutant. The 1453-bp sequence contains an open reading frame (ORF) that predicts a unique 249 amino acid (aa) protein. A Northern blot experiment demonstrated that LYS7 transcription was not regulated by lysine-specific or general aa control mechanisms. To investigate the effects of total loss of...
Topics
- Amino Acid Sequence
- Base Sequence
- Blotting, Northern
- Cloning, Molecular
- Fungal Proteins
- Gene Deletion
- Gene Expression Regulation, Fungal
- Genes, Fungal
- Lysine
- Molecular Chaperones
- Molecular Sequence Data
