Article
Stereoselective catalysis of a retro-Michael reaction by class mu glutathione transferases. Consequences for the internal distribution of products in the active site.
Chemical research in toxicology - 1 Jun 1995
Chen J, Armstrong R N
Abstract excerpt
The reaction of glutathione (GSH) with trans-4-phenyl-3-buten-2-one (PBO) is readily reversible in aqueous solution with an apparent (pH-dependent) equilibrium constant at pH 8 of 6.4 x 10(2) M-1. Two class mu isoenzymes of GSH transferase from rat (M1-1 and M2-2) and two site specific mutants (M1-1/V9I and M2-2/I9V) catalyze the addition of GSH to PBO and the elimination of GSH from the two diastereomeric...
Topics
- Binding Sites
- Butanones
- Catalysis
- Circular Dichroism
- Escherichia coli
- Glutathione
- Glutathione Transferase
- Hydrogen-Ion Concentration
- Isoenzymes
- Kinetics
- Mutation