Article
Enrichment of fetal cells from maternal blood by high gradient magnetic cell sorting (double MACS) for PCR-based genetic analysis.
Prenatal diagnosis - 1 Dec 1994
Büsch J, Huber P, Pflüger E, Miltenyi S, Holtz J, Radbruch A
Abstract excerpt
For simple and effective isolation of fetal cells from peripheral maternal blood, we combined depletion of maternal cells and enrichment of fetal cells by high-gradient magnetic cell separation (MACS). First CD45+ and CD14+ cells were depleted from maternal peripheral blood mononuclear cells by MACS. From the depleted fraction, CD71+ erythroid cells were enriched up to 80 per cent by MACS. This double-MACS'...
Topics
- Antigens, CD
- Antigens, Differentiation, B-Lymphocyte
- Antigens, Differentiation, Myelomonocytic
- Base Sequence
- Cell Separation
- DNA
- Female
- Fetal Blood
- Flow Cytometry
- Fluorescent Antibody Technique