Description of a novel FR1 IgH PCR strategy and its comparison with three other strategies for the detection of clonality in B cell malignancies.

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Article

Description of a novel FR1 IgH PCR strategy and its comparison with three other strategies for the detection of clonality in B cell malignancies.

Leukemia - 1 Mar 1995

Aubin J, Davi F, Nguyen-Salomon F, Leboeuf D, Debert C, Taher M, Valensi F, Canioni D, Brousse N, Varet B

Abstract excerpt

PCR amplification of IgH gene V-D-J junctional variability (IgH PCR) is increasingly replacing Southern analysis for the detection of clonal lymphoid populations in cases presenting diagnostic difficulties. In order to determine the most efficient strategy, we have compared three known methods, using consensus primers against the VH FR3 or FR2 (FR256) regions, or a mix of six primers against the FR1 region...

Topics

Alleles
Antigens, CD
Antigens, Neoplasm
Base Sequence
Blotting, Southern
CD5 Antigens
Clone Cells
Consensus Sequence
DNA Primers
DNA, Neoplasm
Evaluation Studies as Topic

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

False Negative Reactions

False Positive Reactions

Gene Rearrangement, B-Lymphocyte, Heavy Chain

Genes, Immunoglobulin

Humans

Immunoglobulin Heavy Chains

Immunoglobulin J-Chains

Source

Open in PubMed

Identifiers

PMID: 7533868

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7533868-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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