Article
Significantly improved PCR-based clonality testing in B-cell malignancies by use of multiple immunoglobulin gene targets. Report of the BIOMED-2 Concerted Action BHM4-CT98-3936.
Leukemia - 1 Feb 2007
Evans P A S, Pott Ch, Groenen P J T A, Salles G, Davi F, Berger F, Garcia J F, van Krieken J H J M, Pals S, Kluin Ph, Schuuring E, Spaargaren M, Boone E, González D, Martinez B, Villuendas R, Gameiro P, Diss T C, Mills K, Morgan G J, Carter G I, Milner B J, Pearson D, Hummel M, Jung W, Ott M, Canioni D, Beldjord K, Bastard C, Delfau-Larue M H, van Dongen J J M, Molina T J, Cabeçadas J
Abstract excerpt
Polymerase chain reaction (PCR) assessment of clonal immunoglobulin (Ig) and T-cell receptor (TCR) gene rearrangements is an important diagnostic tool in mature B-cell neoplasms. However, lack of standardized PCR protocols resulting in a high level of false negativity has hampered comparability of data in previous clonality studies. In order to address these problems, 22 European laboratories investigated the...
Topics
- Chromosomes, Human, Pair 11
- Chromosomes, Human, Pair 14
- Chromosomes, Human, Pair 18
- Gene Rearrangement
- Genes, Immunoglobulin
- Genotype
- Humans
- Immunoglobulin Heavy Chains