Article
Mutations in the SHR5 gene of Saccharomyces cerevisiae suppress Ras function and block membrane attachment and palmitoylation of Ras proteins.
Molecular and cellular biology - 1 Mar 1995
Jung V, Chen L, Hofmann S L, Wigler M, Powers S
Abstract excerpt
We have identified a gene, SHR5, in a screen for extragenic suppressors of the hyperactive RAS2Val-19 mutation in the budding yeast Saccharomyces cerevisiae. SHR5 was cloned, sequenced, and found to encode a 23-kDa protein not significantly homologous to other proteins in the current data bases. Genetic evidence arguing that Shr5 operates at the level of Ras is presented. We tested whether SHR5, like previously...
Topics
- Acyltransferases
- Amino Acid Sequence
- Base Sequence
- Cell Membrane
- Cloning, Molecular
- DNA Primers
- Epitopes
- Fungal Proteins
- GTP-Binding Proteins
- Genes, Fungal
- Genotype