Article
New polymorphism on platelet glycoprotein IIIa gene recognized by endonuclease Msp I: implications for PlA typing by allele-specific restriction analysis.
Transfusion - 1 Jul 1994
Unkelbach K, Kalb R, Breitfeld C, Santoso S, Kiefel V, Mueller-Eckhardt C
Abstract excerpt
BACKGROUND: Five human platelet alloantigen systems have been shown to result from single base pair substitutions in encoding regions of platelet glycoprotein genes IIIa, Ib, IIb, and Ia. For each of the diallelic systems, at least one restriction enzyme is known to cut only one of the two haplotypes. In the PlA system, restriction endonucleases Nci I and Msp I both recognize the PlA2 allele. STUDY DESIGN AND...
Topics
- Antigens, Human Platelet
- Deoxyribonuclease HpaII
- Deoxyribonucleases, Type II Site-Specific
- Humans
- Mutation
- Platelet Membrane Glycoproteins
- Polymorphism, Genetic
- Polymorphism, Restriction Fragment Length
- Sequence Analysis, DNA