Construction and initial characterization of Escherichia coli strains with few or no intact chromosomal rRNA operons.

doi:10.1128/JB.181.12.3803-3809.1999

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Article

Construction and initial characterization of Escherichia coli strains with few or no intact chromosomal rRNA operons.

Journal of bacteriology - 1 Jun 1999

Asai T, Condon C, Voulgaris J, Zaporojets D, Shen B, Al-Omar M, Squires C, Squires C L

Abstract excerpt

The Escherichia coli genome carries seven rRNA (rrn) operons, each containing three rRNA genes. The presence of multiple operons has been an obstacle to many studies of rRNA because the effect of mutations in one operon is diluted by the six remaining wild-type copies. To create a tool useful for manipulating rRNA, we sequentially inactivated from one to all seven of these operons with deletions spanning the 16S...

Topics

Chromosomes, Bacterial
Escherichia coli
Gene Deletion
Genotype
Mutagenesis
Operon
RNA, Bacterial
RNA, Ribosomal
RNA, Ribosomal, 16S
RNA, Ribosomal, 23S
Restriction Mapping

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Identifiers

PMID: 10368156
DOI: 10.1128/JB.181.12.3803-3809.1999
PMCID: PMC93859

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