Inhibition of Ras/Raf interaction by anti-oncogenic mutants of neurofibromin, the neurofibromatosis type 1 (NF1) gene product, in cell-free systems.

doi:10.1074/jbc.270.48.28834

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Article

Inhibition of Ras/Raf interaction by anti-oncogenic mutants of neurofibromin, the neurofibromatosis type 1 (NF1) gene product, in cell-free systems.

The Journal of biological chemistry - 1 Dec 1995

Mori S, Satoh T, Koide H, Nakafuku M, Villafranca E, Kaziro Y

Abstract excerpt

The neurofibromatosis type 1 (NF1) gene encodes a protein, neurofibromin, containing GTPase-activating protein-related domain (GRD) that stimulates intrinsic GTPase activity of Ras protein. By screening a randomly mutagenized NF1-GRD library in Saccharomyces cerevisiae, we isolated two NF1-GRD mutants (NF201 and NF204) with single amino acid substitutions, which suppress the heat shock-sensitive phenotype of the...

Topics

3T3 Cells
Animals
Cell Line
Cell Transformation, Neoplastic
Cell-Free System
Guanosine Triphosphate
Humans
Kidney
Mice
Mutation
Neurofibromin 1

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Oncogenes

Protein Binding

Protein Serine-Threonine Kinases

Proteins

Proto-Oncogene Proteins

Proto-Oncogene Proteins c-raf

Rats

Source

Open in PubMed

Identifiers

PMID: 7499408
DOI: 10.1074/jbc.270.48.28834

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7499408-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Inhibition of Ras/Raf interaction by anti-oncogenic mutants of neurofibromin, the neurofibromatosis type 1 (NF1) gene product, in cell-free systems.PMID 7499408Read ↗

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