Evidence from genotypic and phenotypic markers that an attenuated line outgrows a virulent one in a mixed population of Leishmania major promastigotes cultured in vitro.

Abstract excerpt

Two cloned lines of Leishmania major promastigotes, one attenuated (CO1H) and one virulent (CO1R), differing in molecular karyotype and expression of the major surface glycoprotein (gp63), were mixed to produce two heterogeneous populations: MP-1 (100 CO1R promastigotes/CO1H promastigote); and MP-2 (10,000 CO1R promastigotes/CO1H promastigote). The mixed populations were cultured for 1 month in vitro in HO-MEM...

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