Article
Population heterogeneity among clones of New World Leishmania species.
Parasitology - 1 Jun 1990
Pacheco R S, Grimaldi G, Momen H, Morel C M
Abstract excerpt
Cell cloning techniques and schizodeme analysis were used to detect mixtures of subpopulations in Leishmania parasites, isolated from humans and a reservoir host. Clones were obtained by plating promastigotes, at limited dilution, on solid medium. The resultant colonies were analysed by the restriction profile of kinetoplast DNA minicircles (schizodeme analysis). The efficiency of plating was around 40-60%, and...
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