Article
Effects of Asp-179 mutations in TEMpUC19 beta-lactamase on susceptibility to beta-lactams.
Antimicrobial agents and chemotherapy - 1 Aug 1995
Vakulenko S B, Tóth M, Taibi P, Mobashery S, Lerner S A
Abstract excerpt
To examine the effect of disruption of the salt bridge (between Arg-164 and Asp-179 [numbering of Ambler et al. (Biochem J. 267:269-272, 1991)]) that anchors the conserved omega-loop in class A beta-lactamases, we obtained mutant enzymes with each of the 19 other amino acid residues replacing Asp-179 in the TEM beta-lactamase encoded by pUC19 and studied the level of resistance to various beta-lactams conferred...
Topics
- Ampicillin Resistance
- Anti-Bacterial Agents
- Cephalosporin Resistance
- Escherichia coli
- Microbial Sensitivity Tests
- Mutation
- Staphylococcus aureus
- Sulfur Radioisotopes
- beta-Lactam Resistance
- beta-Lactamase Inhibitors