Article
Mutation of the conserved Asp-Asp pair impairs the structure, function, and inhibition of CTX-M Class A β-lactamase.
FEBS letters - 1 Dec 2021
Kemp M Trent, Nichols Derek A, Zhang Xiujun, Defrees Kyle, Na Insung, Renslo Adam R, Chen Yu
Abstract excerpt
The Asp233-Asp246 pair is highly conserved in Class A β-lactamases, which hydrolyze β-lactam antibiotics. Here, we characterize its function using CTX-M-14 β-lactamase. The D233N mutant displayed decreased activity that is substrate-dependent, with reductions in kcat /Km ranging from 20% for nitrocefin to 6-fold for cefotaxime. In comparison, the mutation reduced the binding of a known reversible inhibitor by...
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