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Substitution of Met-69 by Ala or Gly in TEM-1 beta-lactamase confer an increased susceptibility to clavulanic acid and other inhibitors.

FEMS microbiology letters - 21 May 2002

Madec Stéphanie, Blin Claudine, Krishnamoorthy Rajagopal, Picard Bertrand, Chaibi El Bachir, Fouchereau-Péron Martine, Labia Roger

Abstract excerpt

In some inhibitor-resistant TEM-derived beta-lactamases, Met-69 is substituted by Leu, Ile or Val. Residue 69 is located in a region of strong structural constraints, at the beginning of H2 alpha-helix, and in the vicinity of B3 and B4 beta-strands. Analysis of the three-dimensional structure of TEM-1 beta-lactamase suggests that alteration of the substrate-binding site can be produced by changes of the size of...

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PMID: 12052544
DOI: 10.1111/j.1574-6968.2002.tb11196.x

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