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Article

Analysis of immunoglobulin VH gene repertoire by an anchored PCR-ELISA.

Annals of the New York Academy of Sciences - 29 Sept 1995

Rassenti L Z, Kohsaka H, Kipps T J

Abstract excerpt

We developed a novel technique to analyze the relative concentration of the expressed immunoglobulin (Ig) VH genes using an enzyme-linked immunosorbent assay (ELISA). Expressed Ig cDNA are amplified via anchored PCR and then subjected to a "nested PCR" reaction that attaches biotin to the 5' end of the antisense strand. This allows us to tether the antisense strand of PCR products onto avidin-coated ELISA plates....

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Source

Identifiers

PMID: 7486567
DOI: 10.1111/j.1749-6632.1995.tb55866.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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