Analysis of immunoglobulin VH gene repertoire by an anchored PCR-ELISA.

doi:10.1111/j.1749-6632.1995.tb55866.x

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Article

Analysis of immunoglobulin VH gene repertoire by an anchored PCR-ELISA.

Annals of the New York Academy of Sciences - 29 Sept 1995

Rassenti L Z, Kohsaka H, Kipps T J

Abstract excerpt

We developed a novel technique to analyze the relative concentration of the expressed immunoglobulin (Ig) VH genes using an enzyme-linked immunosorbent assay (ELISA). Expressed Ig cDNA are amplified via anchored PCR and then subjected to a "nested PCR" reaction that attaches biotin to the 5' end...

Topics

Adult
Antibodies, Monoclonal
Antibody Diversity
B-Lymphocytes
Base Sequence
Clone Cells
Colorimetry
DNA Mutational Analysis
DNA, Complementary
Digoxigenin
Enzyme-Linked Immunosorbent Assay
Gene Rearrangement, B-Lymphocyte, Heavy Chain

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Genes, Immunoglobulin

Humans

Immunoglobulin Heavy Chains

Immunoglobulin Variable Region

Leukemia, Lymphocytic, Chronic, B-Cell

Molecular Sequence Data

Source

Open in PubMed

Identifiers

PMID: 7486567
DOI: 10.1111/j.1749-6632.1995.tb55866.x

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-7486567-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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