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Article

An efficient helper-virus-free method for rescue of recombinant paramyxoviruses and rhadoviruses from a cell line suitable for vaccine development.

Journal of virological methods - 1 Jul 2006

Witko Susan E, Kotash Cheryl S, Nowak Rebecca M, Johnson J Erik, Boutilier Lee Anne C, Melville Krista J, Heron Sannyu G, Clarke David K, Abramovitz Aaron S, Hendry R Michael, Sidhu Mohinder S, Udem Stephen A, Parks Christopher L

Abstract excerpt

Recovery of recombinant, negative-strand, nonsegmented RNA viruses from a genomic cDNA clone requires a rescue system that promotes de novo assembly of a functional ribonucleoprotein (RNP) complex in the cell cytoplasm. This is accomplished typically by cotransfecting permissive cells with multiple plasmids that encode the positive-sense genomic RNA, the nucleocapsid protein (N or NP), and the two subunits of the...

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PMID: 16569439
DOI: 10.1016/j.jviromet.2006.02.006

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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