Article
Severe phenotype in mice with termination mutation in exon 2 of cystic fibrosis gene.
Somatic cell and molecular genetics - 1 May 1995
Hasty P, O'Neal W K, Liu K Q, Morris A P, Bebok Z, Shumyatsky G B, Jilling T, Sorscher E J, Bradley A, Beaudet A L
Abstract excerpt
Mice with a termination codon mutation in exon 2 of the cystic fibrosis (CF) gene were generated using homologous recombination in embryonic stem cells. Animals homozygous for the mutant allele display a severe intestinal phenotype similar to that previously reported for CF mutant mice. The null nature of this allele was demonstrated by the absence of detectable wild-type mRNA, by the absence of detectable CFTR...
Topics
- Animals
- Base Sequence
- Chlorides
- Cystic Fibrosis
- Cystic Fibrosis Transmembrane Conductance Regulator
- DNA
- Exons
- Immunohistochemistry
- Intestinal Mucosa
- Ion Transport
- Mice