Article
Cloning and molecular analysis of the dihydrofolate reductase gene from Lactococcus lactis.
Applied and environmental microbiology - 1 Feb 1995
Leszczyńska K, Bolhuis A, Leenhouts K, Venema G, Cegłowski P
Abstract excerpt
The Lactococcus lactis gene encoding trimethoprim resistance has been cloned and expressed in Escherichia coli and Bacillus subtilis. Several lines of evidence indicate that the cloned gene encodes dihydrofolate reductase (DHFR). (i) It fully complements the fol "null" mutation in E. coli. (ii) N...
Topics
- Amino Acid Sequence
- Bacillus subtilis
- Base Sequence
- Cloning, Molecular
- DNA, Bacterial
- Drug Resistance, Microbial
- Escherichia coli
- Gene Expression
- Genes, Bacterial
- Lactococcus lactis
- Molecular Sequence Data
- Phenotype
- Sequence Homology, Amino Acid
- Tetrahydrofolate Dehydrogenase
- Transcription, Genetic
- Trimethoprim