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Article

Cloning and molecular analysis of the dihydrofolate reductase gene from Lactococcus lactis.

Applied and environmental microbiology - 1 Feb 1995

Leszczyńska K, Bolhuis A, Leenhouts K, Venema G, Cegłowski P

Abstract excerpt

The Lactococcus lactis gene encoding trimethoprim resistance has been cloned and expressed in Escherichia coli and Bacillus subtilis. Several lines of evidence indicate that the cloned gene encodes dihydrofolate reductase (DHFR). (i) It fully complements the fol "null" mutation in E. coli. (ii) Nucleotide sequencing of the cloned fragment revealed the presence of one open reading frame encoding a protein that...

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Source

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Identifiers

PMID: 7574597
DOI: 10.1128/aem.61.2.561-566.1995
PMCID: PMC167319

Genes

DHFR

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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