Article
[Mutations in Escherichia coli pmp and htpR genes stabilize the products of foreign gene expression].
Molekuliarnaia biologiia - 1 Jan 2000
Kliachko E V, Lysenko E S, Eremashvili M R, Kozlov Iu I, Strongin A Ia
Abstract excerpt
The half lives of mRNA for Escherichia coli chloramphenicol-acetyltransferase, Bacillus amyloliquefaciens alpha-amylase and human leucocyte interferon were measured in E. coli cells by molecular RNA.DNA hybridization. The effect of mutation in pnp gene, coding polynucleotide phosphorylase, on the stability of these mRNA was studied. The half life of interferon mRNA increases from 25 to 90 s in the pnp mutant,...
Topics
- Cloning, Molecular
- Escherichia coli
- Gene Expression Regulation
- Genes, Bacterial
- Half-Life
- Mutation
- Nucleic Acid Hybridization
- Plasmids
- RNA, Messenger
- Recombinant Proteins