Transposition of DNA fragments flanked by two inverted Tn1 sequences: translocation of the plasmid RP4::Tn1 region harboring the Tcr marker.

doi:10.1016/0378-1119(83)90108-7

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Article

Transposition of DNA fragments flanked by two inverted Tn1 sequences: translocation of the plasmid RP4::Tn1 region harboring the Tcr marker.

Gene - 1 Jan 2000

Dobritsa A P, Ivanova Z A, Fedoseeva V B

Abstract excerpt

We have demonstrated the possibility of transposition of the plasmid RP4::Tn1 fragment (21.2 kb) carrying the tetracycline resistance (Tcr) gene and flanked by two Tn1 copies. The new transposon, designated Tn1756, bears lethal genes that kill host cells. Therefore, its transposition can only be...

Topics

Base Sequence
Crosses, Genetic
DNA Restriction Enzymes
DNA Transposable Elements
DNA, Bacterial
Escherichia coli
Genes, Bacterial
Genes, Lethal
Genotype
Microscopy, Electron
Phenotype
Plasmids

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