Article
A genetic system for analysis of staphylococcal nuclease.
Gene - 1 Jan 2000
Shortle D
Abstract excerpt
The gene encoding staphylococcal nuclease (strain Foggi) has been isolated, and its complete nucleotide sequence determined. When inserted into a derivative of plasmid pBR322, the nuc gene is expressed in Escherichia coli at a low level, and nuclease activity in individual colonies is readily assayed by a replica-plating chromogenic test. A set of plasmids with BamHI-linker "bracketed" deletions spanning the nuc...
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