Article
Proteolytic response to the expression of an abnormal beta-galactosidase in Escherichia coli.
Applied microbiology and biotechnology - 1 Jun 1992
Kosinski M J, Rinas U, Bailey J E
Abstract excerpt
Because induction of proteolytic activity and stress-response proteins can significantly affect expression levels in recombinant Escherichia coli, the influence of low-level expression of a mutant beta-galactosidase was investigated. A single copy of the well-characterized CSH11 mutant of the lacZ gene was integrated into the chromosome. Induction of expression of the mutant beta-galactosidase caused a measurable...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
