Proteolytic response to the expression of an abnormal beta-galactosidase in Escherichia coli.

doi:10.1007/BF00210989

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Article

Proteolytic response to the expression of an abnormal beta-galactosidase in Escherichia coli.

Applied microbiology and biotechnology - 1 Jun 1992

Kosinski M J, Rinas U, Bailey J E

Abstract excerpt

Because induction of proteolytic activity and stress-response proteins can significantly affect expression levels in recombinant Escherichia coli, the influence of low-level expression of a mutant beta-galactosidase was investigated. A single copy of the well-characterized CSH11 mutant of the lac...

Topics

Adenosine Triphosphate
Biotechnology
Escherichia coli
Gene Expression
Heat-Shock Proteins
Mutation
Peptide Hydrolases
Recombinant Proteins
Temperature
beta-Galactosidase

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