Article
Identifying a large number of high-yield genes in rice by pedigree analysis, whole-genome sequencing, and CRISPR-Cas9 gene knockout.
Proceedings of the National Academy of Sciences of the United States of America - 7 Aug 2018
Huang Ju, Li Jing, Zhou Jun, Wang Long, Yang Sihai, Hurst Laurence D, Li Wen-Hsiung, Tian Dacheng
Abstract excerpt
Repeated artificial selection of a complex trait facilitates the identification of genes underlying the trait, especially if multiple selected descendant lines are available. Here we developed a pedigree-based approach to identify genes underlying the Green Revolution (GR) phenotype. From a pedigree analysis, we selected 30 cultivars including the "miracle rice" IR8, a GR landmark, its ancestors and descendants,...
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