Four haplotype-resolved genome assemblies and a reference-free 66-haplotype pangenome graph for Cannabis sativa.

doi:10.1038/s41597-026-07806-y

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Article

Four haplotype-resolved genome assemblies and a reference-free 66-haplotype pangenome graph for Cannabis sativa.

Scientific data - 26 Aug 2026

Pike Brett, Gonçalves da Silva Anders, Terán Wilson

Abstract excerpt

We present 4 haplotype-resolved, chromosome scale diploid assemblies of cannabis, assembled from ONT R9.4.1 reads via a novel pipeline based on Hi-C phasing. These assemblies, while low in QV, offer contiguity and genic content comparable to recent HiFi assemblies. Along with a trio-binned assembly previously produced by us and 56 haplotypes selected from the Salk Institute Cannabis Pangenome project, we use...

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PMID: 42649226
DOI: 10.1038/s41597-026-07806-y
PMCID: PMC13518974

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