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Article

A new NMR protocol for estimating protein side chain rotameric preferences using 1H relaxation and J couplings.

Journal of magnetic resonance (San Diego, Calif. : 1997) - 1 Sept 2026

Goel Shaista, Mak Kevin Y L, Guan Sonja Z T, Dumont Thomas W, Zuiderweg Erik R P, Case David A, Hwang Peter M

Abstract excerpt

Herein we demonstrate the utility of measuring initial 1H NMR relaxation rates for determining protein side chain mobility in a human Pin1 WW domain thermostable mutant. To accomplish this, a 1H spinlock field or TOCSY element is appended to the beginning of any multidimensional NMR experiment beginning on 1H. This initial assessment is essential for determining the appropriate side chain motional model for...

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PMID: 42284882
DOI: 10.1016/j.jmr.2026.108100

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