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Article

Development and application of a fast and efficient CRISPR/Cas12f -based genetic toolkit in Bacillus cereus GW-01.

Journal of microbiological methods - 1 Aug 2026

Wei Enting, Tang Ying, Lei Ying, Liu Shan, Xu Bowen, Du Baiyi, Wang Yanchun, Liao Ying, Wang Yanan, Zhao Jiayuan

Abstract excerpt

Bacillus cereus GW-01, an efficient degrader of β-cypermethrin (β-CY), has a high safety profile and probiotic potential for regulating intestinal flora and fermented foods, which is difficult to genetically engineer for modification due to its restrictive modification system. This study successfully developed a CRISPR/enCas12f-based genome editing system, first selecting the plcR gene for proof-of-concept...

Read the complete abstract on PubMed

Topics

Bacillus cereus
Clustered Regularly Interspaced Short Palindromic Repeats
Bacterial Proteins
CRISPR-Associated Proteins
Mutation

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Probiotics

Pyrethrins

Gene Editing

Source

Identifiers

PMID: 42269938
DOI: 10.1016/j.mimet.2026.107584

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