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A fluorescence-based high-content imaging workflow for multi-parametric lipid droplet phenotyping via Oil Red O fluorescence.

Methods (San Diego, Calif.) - 1 Oct 2026

Yan Shaojun, Yao Yuqin, Liu Congying, Zhu Hui, Wu Xiaoyan, Lyu Chunming, Yang Yang

Abstract excerpt

Progression of Metabolic-associated Fatty Liver Disease (MAFLD) involves excessive accumulation of intrahepatic lipid droplets (LDs). However, cost-effective, high-throughput imaging for detailed, multi-parametric LDs quantification at the single-cell level remains difficult. MATERIALS AND METHODS: Here, we established a high-content screening (HCS) platform that utilizes the fluorescence of Oil Red O (ORO) to...

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Azo Compounds
Fatty Liver
High-Throughput Screening Assays
Lipid Droplets
Non-alcoholic Fatty Liver Disease

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Optical Imaging

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PMID: 42259461
DOI: 10.1016/j.ymeth.2026.06.002

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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