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Article

A one-pot RPA-T7/crDNA-CRISPR/Cas13a assay for portable and ultrasensitive detection of Helicobacter pylori and clarithromycin resistance mutations.

International journal of biological macromolecules - 1 Aug 2026

Han Yongjun, Wu Hong, Gao Shuibo, Wang Youping, Ma Qiuling

Abstract excerpt

The high global prevalence of Helicobacter pylori (H. pylori) infection and rising clarithromycin resistance demand rapid, sensitive, point-of-care diagnostic tools. Although CRISPR/Cas systems have revolutionized nucleic acid detection, challenges such as unstable pre-synthesized crRNA and multi-step procedures limit their practical application. Here, we report a one-pot assay, termed RT-CRISPR...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 42251880
DOI: 10.1016/j.ijbiomac.2026.152949

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

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