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Asy-RPA/PCR combined with One-crRNA-CRISPR/Cas12a for simultaneous detection of multiple Clarithromycin resistance mutations in Helicobacter pylori.

Nanomedicine : nanotechnology, biology, and medicine - 1 Jun 2026

Liu Yanghe, Chen Shiyue, Zhang Chunlei, Xue Hui, Abubakar Muhammad Usman, Yin Sijie, Yang Xincheng, Fan Boyue, Tai Ping, Xiong Mengqiu, Li Jingjing, He Bangshun

Abstract excerpt

METHODS: Genetic testing for Clarithromycin resistance-associated single-nucleotide variations (SNVs) in H. pylori could be applied for formulating individual eradication plan. In this study, we integrated asymmetric recombinase polymerase amplification (Asy-RPA) with a single crRNA for CRISPR/Cas12a-designated the ARoRC system-to circumvent protospacer adjacent motif (PAM) dependency. RESULTS: The ARoRC platform...

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Share to Anti-Bacterial Agents
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 41962729
DOI: 10.1016/j.nano.2026.102942

Genes

PAM

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

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