Article
Hybrid sequencing reveals incompleteness of the H37Rv reference genome and highlights lineage-specific genomic divergence in Mycobacterium tuberculosis.
Microbial genomics - 1 Jun 2026
Chen Haiting, Li Xiangchen, Chen Ruiqi, Zhang Mingwu, Zhu Yelei, Wu Kunyang, Lu Yewei, Wang Xiaomeng, Che Yang, Liu Zhengwei
Abstract excerpt
Background. The Mycobacterium tuberculosis H37Rv reference genome (Lineage 4), sequenced in 1998, may be incomplete and inaccurate due to earlier technology. Moreover, its use is problematic in regions like East Asia where the dominant Beijing lineage (Lineage 2) is prevalent, as it may introduce biases due to lineage-specific genetic differences.Methods. We performed hybrid sequencing to generate high-quality...
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