Tetra-ARMS-PCR assay development for genotyping of AGT rs699 T/C polymorphism, its comparison with PCR-RFLP and application in a case-control association study of cardiovascular disease patients.

doi:10.1080/15257770.2023.2181972

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Tetra-ARMS-PCR assay development for genotyping of AGT rs699 T/C polymorphism, its comparison with PCR-RFLP and application in a case-control association study of cardiovascular disease patients.

Nucleosides, nucleotides & nucleic acids - 1 Jan 2023

Hussain Misbah, Khan Haq Nawaz, Abbas Shahid, Ali Ansar, Aslam Muhammad Naeem, Awan Fazli Rabbi

Abstract excerpt

Genetic variations in the AGT gene play a significant role in controlling the plasma concentration of angiotensinogen (precursor protein of bioactive octapeptide angiotensin II) and the efficacy of antihypertensive drugs. In the current study, Tetra-Amplification Refractory Mutation System-Polymerase Chain Reaction (T-ARMS-PCR) was developed for genotyping of AGT rs699 T/C polymorphism and validated through...

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PMID: 36815563
DOI: 10.1080/15257770.2023.2181972

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