Article
T-ARMS PCR genotyping of SNP rs445709131 using thermostable strand displacement polymerase.
BMC research notes - 15 Feb 2018
Alyethodi Rafeeque R, Singh Umesh, Kumar Sushil, Alex Rani, Deb Rajib, Sengar Gyanendra S, Raja T V, Prakash B
Abstract excerpt
OBJECTIVES: In a recent publication, we reported the successful use of tetra primer-amplification refractory mutation system based polymerase chain reaction (T-ARMS-PCR) for genotyping of rs445709131-SNP responsible for the bovine leukocyte adhesion deficiency (BLAD) in cattle. The SNP is characterized by higher GC content of the surrounding region, hence, the previous protocol utilized dimethyl sulfoxide as PCR...
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