Comparative transcriptomic profiling of high-yielding Saccharopolyspora pogona mutants generated by consecutive N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis.

Abstract excerpt

Butenyl-spinosyn is a highly effective macrolide insecticide produced by Saccharopolyspora pogona, but its low yield remains a major bottleneck for large-scale industrial application. In this study, a strategy based on consecutive N-methyl-N'-nitro-N-nitrosoguanidine (NTG) mutagenesis was employed, leading to the isolation of a genetically stable high-yielding mutant strain, ASAGF9-86, which produced 134.8 mg/L...

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