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Article

A streamlined, nanopore-compatible 5PSeq protocol for rapid phenotypic antimicrobial sensitivity testing.

Cell reports methods - 23 Mar 2026

Liu Honglian, Huch Susanne, Hull Ryan, Garcia Fabricio Romero, Nersisyan Lilit, Yin Xiushan, Chen Wei-Hua, Du Juan, Pelechano Vicent

Abstract excerpt

Antimicrobial resistance (AMR) poses a significant threat to public health. Rapid and accurate antimicrobial sensitivity testing is essential to guide effective treatment. Here, we present "simplified 5PSeq" (s5PSeq), a streamlined protocol for profiling 5' monophosphorylated (5'P) mRNA degradation intermediates that reflect ribosome dynamics in vivo. By capturing antibiotic-induced, context-specific ribosome...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 41825450
DOI: 10.1016/j.crmeth.2026.101327
PMCID: PMC13030957

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

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