Article
Integrating SMRT and Bulk RNA Sequencing With Metabolic Phenotyping to Examine Reduced Skeletal Muscle Mitochondrial Respiration in Type 2 Diabetes.
Diabetes - 1 Mar 2026
Schön Martin, Oehler Daniel, Yurchenko Iryna, Lang Alexander, Trinks Nina, Dewidar Bedair, Mastrototaro Lucia, Zaharia Oana P, Bódis Kálmán B, Karusheva Yanislava, Toledo Frederico G S, Burkart Volker, Granata Cesare, Westenfeld Ralf, Polzin Amin, Kelm Malte, Wagner Robert, Roden Michael, Szendroedi Julia
Abstract excerpt
Recent advances in RNA sequencing (RNA-seq) techniques allow the identification of tissue-specific alternative splicing and can thereby provide new insights into molecular mechanisms of energy metabolism. Full-length transcriptomics based on single-molecule real-time sequencing (SMRT-seq) enable precise detection of isoforms with 99% accuracy in an unbiased manner. In this proof-of-concept study, we integrated...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.