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Article

Protocol for 3D-guided sectioning and deep cell phenotyping via light sheet imaging and 2D spatial multiplexing.

STAR protocols - 20 Mar 2026

Bigott Kevin, Schoppel Victoria H, Martinez-Osuna Manuel, Osinski Leon, Tiveron Marie-Catherine, Barleben Daniel, Bornemann Simon F, Cremer Harold, Herbel Christoph, Bosio Andreas, Jungblut Melanie

Abstract excerpt

Spatial analysis of cells and their microenvironment within tissues enhances our understanding of biological processes. Ideally, a broad range of biomolecules should be analyzed in large 3D tissue specimens at subcellular resolution. Here, we present a protocol to identify and extract target sections from previously cleared tissues. We describe steps for combining 3D light sheet imaging and subsequent 3D-guided...

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Identifiers

PMID: 41456277
DOI: 10.1016/j.xpro.2025.104296
PMCID: PMC12796729

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

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