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Article

A novel one-tube nested fluorescence melting curve analysis for rapid detection of drug-resistant Mycobacterium tuberculosis.

BMC microbiology - 20 Dec 2025

Chang Yingchao, Liu Gaowen, Li Xiaofei, Zhang Mi, Diao Tingting, Yang Cuixian, Wu Xinlin, Tu Yungui, Zhang Hongji, Zhou Chao, Lei Pei, Liu Li, Xia Xueshan, Feng Yue

Abstract excerpt

BACKGROUND: Rapidly detecting drug-resistant Mycobacterium tuberculosis (MTB) is essential for effective tuberculosis (TB) treatment. We have developed a one-tube nested fluorescence melting curve assay (OTN-FMCA) that allows rapid and simultaneous detection of resistance to rifampicin, isoniazid, fluoroquinolones, and bedaquiline. RESULTS: The OTN-FMCA uses nested PCR and locked nucleic acid (LNA)-modified...

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Identifiers

PMID: 41420205
DOI: 10.1186/s12866-025-04601-4
PMCID: PMC12859952

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