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Article

Real-time PCR assay for robust detection and global surveillance of the Mycobacterium tuberculosis Haarlem genotype.

Scientific reports - 11 Dec 2025

Tkachuk Daria, Vyazovaya Anna, Kostyukova Irina, Couvin David, Zhdanova Svetlana, Bakuła Zofia, Jiao Weiwei, Solovieva Natalia, Eliseev Platon, Badleeva Maria, Kobesov Nikolai, Dzagoev Valentin, Wuyep Valentine B, Ikeh Eugene I, Nayzabaeva Dinara, Kondratov Ilya, Bezruchko Mikhail, Chekrygin Sergei, Polev Dmitrii, Gorokhov Ivan, Sergeev Grigorii, Chilikina Alena, Pasechnik Oksana, Schwartz Yakov, Zhuravlev Viacheslav, Ogarkov Oleg, Mariandyshev Andrei, Shen Adong, Jagielski Tomasz, Singh Urvashi, Valcheva Violeta, Skiba Yuriy, Mokrousov Igor

Abstract excerpt

The Haarlem genotype is a significant yet understudied part of the Euro-American lineage of Mycobacterium tuberculosis, characterized by unique pathogenetic features. Spoligotyping is a primary method for its detection, but it is not suitable for isolates with long blocks of deleted spacers. We have developed a simple and robust method to detect the Haarlem genotype. The real-time PCR (RT-PCR) assay with LNA...

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PMID: 41381572
DOI: 10.1038/s41598-025-27556-y
PMCID: PMC12699029

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