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Article

A β-lactamase inhibitory protein mutant displays high potency and a broad inhibition profile due to an altered binding mode with β-lactamases.

The Journal of biological chemistry - 1 Dec 2025

Rivera Paola, Lu Shuo, Ngango Dignite, Sankaran Banumathi, Venkataram Prasad Bidadi, Palzkill Timothy

Abstract excerpt

β-lactamase enzymes inactivate β-lactam antibiotics, leading to drug resistance. The β-lactamase inhibitory protein (BLIP) is a naturally occurring inhibitor of β-lactamases, with inhibition constants (Ki) ranging from picomolar to micromolar values. For example, BLIP inhibits CTX-M-14 β-lactamase with a Ki of 330 nM, whereas the Ki for CTX-M-15 is 3 nM, despite CTX-M-14 and CTX-M-15 sharing 83% sequence...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 41135675
DOI: 10.1016/j.jbc.2025.110850
PMCID: PMC12666564

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