Article
PCR-CRISPR/Cas12a-based fluorescence and lateral flow dipstick platforms for efficient screening of CD71 biallelic mutants.
Analytica chimica acta - 22 Nov 2025
Nie Yuxin, Wang Wanjie, Wang Nan, Yuan Maosha, Huang Lei, Sun Yaru, Li Kui, Liu Zhiguo, Mu Yulian
Abstract excerpt
CRISPR/Cas9 technology plays a pivotal role in gene editing and has been extensively utilized in gene function studies, disease modeling, and gene therapy. However, efficient and accurate detection of CRISPR/Cas9-induced mutants remains a challenge due to the complexity, time-consuming nature, an...
Topics
- CRISPR-Cas Systems
- Humans
- Receptors, Transferrin
- Polymerase Chain Reaction
- Mutation
- Antigens, CD
- Alleles
- Fluorescence
- CRISPR-Associated Proteins
- Bacterial Proteins
- Endodeoxyribonucleases