Article
Mutations in active surface sites of NtGGPPS1 enhance carotenoid biosynthesis and drought resistance in Nicotiana tabacum.
BMC plant biology - 2 Oct 2025
Song Shanshan, Gao Yulong, Fan Puqing, Lin Faming, Zhou Houliang, Li Zhiyang, Liu Hang, Su Hongyi, Zhong Qiang, Liu Zhuonan, Ren Xinru, Wang Xiaoran, Shi Yongchun, Zhang Yu, Zhang Xiaoquan, Wang Ran
Abstract excerpt
BACKGROUND: Amino acids on the surface of enzymes play a vital role in their catalytic activity and functional properties, influencing enzymatic stability and inducing conformational changes. RESULTS: In this research, we demonstrate that surface mutations in geranylgeranyl diphosphate synthase1 from Nicotiana tabacum (NtGGPPS1) improve carotenoid biosynthesis and increase drought tolerance. We introduced three...
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