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Profiling of Macrophage Polarization Using Automated Enzyme-Linked Immunosorbent Assay.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2026

Loevenich Simon, Nakstad Vu To, Trøen Hanne Hein, Sigfúsdóttir Margrét Sylvía, Sletta Kristine, Nærdal Guro Kruge, Sletta Håvard, Klinkenberg Geir, Haslene-Hox Hanne, Visnes Torkild

Abstract excerpt

Macrophage activation has emerged as a key area in immunology, in which they are polarized to adopt different functional phenotypes in response to various environmental signals. Here, we describe the procedure of isolating human primary CD14+ monocytes and polarization toward M1 (pro-inflammatory) and M2 (anti-inflammatory) phenotypes. Relevant cytokine levels can be detected by manual and automated enzyme-linked...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 41028255
DOI: 10.1007/978-1-0716-4734-9_1

Genes

CD14

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 8 Sept 2026.

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