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Probe-Based qPCR Workflow Using Fast Nucleic Acid Sampling and Molecular Genotyping in a Dioecious Plant.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2025

Torres Anthony, Gaudino Reginald

Abstract excerpt

This chapter presents a protocol for probe-based quantitative polymerase chain reaction (qPCR) genotyping, focusing on male-specific genotype identification in plant samples. DNA is isolated from raw tissue or FTA cards and subjected to a preamplification enrichment reaction. The diluted product is then used as input for qPCR detection, employing specific probes to identify the presence or absence of the target...

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PMID: 40580289
DOI: 10.1007/978-1-0716-4642-7_9

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