Article
qPCR Genotyping of Polyploid Species.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2023
Wang Haiyan, Dang Jiangbo, Guo Qigao, Liang Guolu
Abstract excerpt
A simple and cost-effective method for genotyping polyploid plants using quantitative PCR (qPCR) is described in this chapter. There is no additional operation, only simultaneous amplification of alleles and reference sequences with constant copy number in the genome. The qPCR genotyping can detect the genotypes of important traits in polyploid plants without whole genome sequencing data.
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