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Probe-Based RT-qPCR Workflow for Dual Interrogation of Virus/Viroid Genotype in Molecular Diagnostics Multiplex.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2025

Torres Anthony, Gaudino Reginald

Abstract excerpt

This chapter presents a protocol for dual interrogation reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) genotyping, a rapid, high-throughput, and economical molecular diagnostic approach for the simultaneous detection of viroid and virus genotypes in plant samples. The method utilizes crude nucleic acid extracts from raw tissue or FTA cards, streamlining sample preparation and enabling...

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PMID: 40580290
DOI: 10.1007/978-1-0716-4642-7_10

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