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Streamlined whole-genome genotyping through NGS-enhanced thermal asymmetric interlaced (TAIL)-PCR.

Plant communications - 9 Sept 2024

Zhao Sheng, Wang Yue, Zhu Zhenghang, Chen Peng, Liu Wuge, Wang Chongrong, Lu Hong, Xiang Yong, Liu Yuwen, Qian Qian, Chang Yuxiao

Abstract excerpt

Whole-genome genotyping (WGG) stands as a pivotal element in genomic-assisted plant breeding. Nevertheless, sequencing-based approaches for WGG continue to be costly, primarily owing to the high expenses associated with library preparation and the laborious protocol. During prior development of foreground and background integrated genotyping by sequencing (FBI-seq), we discovered that any sequence-specific primer...

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PMID: 38845197
DOI: 10.1016/j.xplc.2024.100983
PMCID: PMC11412933

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