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Article

Digital PCR-Based Genotyping: A Precision Approach to HCMV Drug Resistance.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2025

Milavec Mojca, Cvelbar Tašja, Bogožalec Košir Alexandra

Abstract excerpt

The genotyping workflow described uses digital PCR (dPCR) to detect and quantify drug resistance mutations in human cytomegalovirus (HCMV). The method focuses on the detection and quantification of three common mutations in the UL97 gene at codons 460, 594, and 595, which are responsible for the majority of ganciclovir-resistant clinical isolates. The dPCR approach offers high sensitivity and accuracy, making it...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 40580282
DOI: 10.1007/978-1-0716-4642-7_2

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